Atlanta United FC

Colorado Rapids | FC Dallas | Houston Dynamo | LA Galaxy | Los Angeles FC | Minnesota United FC | Nashville SC | Portland Timbers | Real Salt Lake | San Jose Earthquakes | Seattle Sounders FC | Sporting Kansas City | Vancouver Whitecaps FC

Eastern Conference:
Atlanta United FC | Chicago Fire FC | FC Cincinnati | Columbus Crew SC | D.C. United | Inter Miami | Montreal Impact | New England Revolution | New York City FC | New York Red Bulls | Orlando City SC | Philadelphia Union | Toronto FC

Future clubs:
Austin FC (2021) | Sacramento Republic (2023) | Charlotte (2022) | St. Louis (2023)

Former clubs:
Miami Fusion FC | Tampa Bay Mutiny | Chivas USA

Related:
eMLS | MLS Cup | MLS Cup Playoffs | MLS Is Back Tournament