Eastern Conference: Northeast Division: Connecticut | Black Rock | Boston Bolts | GPS Portland Phoenix | Manhattan | Seacoast United Phantoms | Westchester Flames | Western Mass Pioneers
Mid Atlantic Division: Cedar Stars Rush | Evergreen FC | FA Euro | Lehigh Valley United | Long Island Rough Riders | New York Red Bulls U23 | Ocean City Nor'easters | Reading United AC
South Atlantic Division: Lionsbridge FC | North Carolina FC U23 | North Carolina Fusion U-23 | Tobacco Road FC | Tri-Cities Otters | Virginia Beach United FC | Wake FC
Southern Conference: Deep South Division: Charlotte Eagles | Dalton Red Wolves SC | Discoveries SC | Peachtree City MOBA | SC United Bantams | South Georgia Tormenta 2
Southeast Division: Daytona SC | FC Miami City | Florida Elite SA | Lakeland Tropics | Sarasota Metropolis FC | The Villages SC | Treasure Coast Tritons | Weston FC
Mid South Division: AHFC Royals | Brazos Valley Cavalry FC | Corpus Christi FC | Houston FC | Mississippi Brilla | Texas United
Central Conference: Great Lakes Division: Chicago FC United | Cincinnati Dutch Lions | Dayton Dutch Lions | Flint City Bucks | West Virginia Alliance FC
Heartland Division: Des Moines Menace | Green Bay Voyageurs | Kaw Valley FC | St. Louis Lions | Thunder Bay Chill | WSA Winnipeg
Western Conference: Northwest Division: Calgary Foothills FC | Lane United FC | Portland Timbers U23 | Seattle Sounders FC U-23 | TSS FC Rovers | Victoria Highlanders
Mountain Division: Albuquerque Sol FC | Colorado Pride Switchbacks U23 | Ogden City SC | Park City Red Wolves
Southwest Division: FC Golden State Force | Orange County SC U-23 | San Diego Zest FC | San Francisco City FC | San Francisco Glens SC | Santa Cruz Breakers FC | Southern California Seahorses | Ventura County Fusion
