| Northeast Region
Atlantic-Blue Conference:
Brooklyn Italians | Elm City Express | Greater Lowell United | New York Cosmos B | TSF FC
Atlantic-White Conference:
Boston City | Hartford City | Kingston Stockade | New York Athletic Club | Rhode Island Reds | Seacoast United Mariners
Keystone Conference:
Atlantic City FC | Buxmont Torch | Electric City Shock | FC Monmouth | FC Motown | Hershey FC | Junior Lone Star FC | New Jersey Copa | West Chester United
Mid-Atlantic Conference:
Charlottesville Alliance | FC Baltimore | FC Frederick | Legacy 76 | Northern Virginia United | Virginia Beach City
South Region
Heartland Conference:
Demize NPSL | FC Wichita | Little Rock Rangers | Ozark FC | Saint Louis Club Atletico | Tulsa Athletic
Lone Star Conference:
FC Brownsville | Fort Worth Vaqueros | Houston Dutch Lions | Houston Regals | Katy 1895 FC | Laredo Heat | Midland-Odessa FC | Shreveport Rafters | Tyler FC
Southeast Conference:
Asheville City | Atlanta Silverbacks | Chattanooga FC | Georgia Revolution | Greenville FC | Inter Nashville | Knoxville Force | New Orleans Jesters
Sunshine Conference:
Boca Raton FC | Cape Coral Hurricanes | Jacksonville Armada | Miami FC 2 | Miami United | Naples United | Palm Beach United | Storm FC
Midwest Region
East Conference:
Erie Commodores | FC Buffalo | Fort Pitt Regiment | Rochester Lancers | Syracuse FC
Great Lakes Conference:
AFC Ann Arbor | Detroit City FC | Grand Rapids FC | FC Columbus | FC Indiana | Kalamazoo FC | Milwaukee Torrent
North Conference:
Dakota Fusion | Duluth FC | La Crosse Aris | Med City FC | Minneapolis City | Minnesota TwinStars | Sioux Falls Thunder | Viejos Son Los Trapos FC
West Region
Golden Gate Conference:
Academica SC | CD Aguiluchos USA | East Bay FC Stompers | El Farolito SC | FC Davis | Napa Valley 1839 | Sacramento Gold | Sonoma County Sol
Northwest Conference:
Kitsap SC | FC Mulhouse Portland | OSA FC | PDX FC | Pierce County FC | Spokane SC Shadow
Southwest Conference:
Albion SC Pros | City of Angels FC | Deportivo Coras USA | FC Arizona | FC Golden State | Orange County FC | Oxnard Guerreros | Temecula FC
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